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. Author manuscript; available in PMC: 2017 Jun 5.
Published in final edited form as: Eur Urol. 2011 Jan 12;59(5):734–744. doi: 10.1016/j.eururo.2010.12.038

Fig. 3.

Fig. 3

Apoptotic response of renal cancer cells to quinazolines. Human renal cancer 786-0 cells were treated with doxazosin or DZ-50 for increasing periods of time (0–48 h) and (A) cleavage of caspase 8 and caspase 3 was determined by Western blotting using the respective antibodies. (B) Data from evaluation of caspase-8-specific activity in 786-0 cells after treatment with DZ-50 (DZ; 10 μM), doxazosin (DOX; 25 μM), Trail (100 ng/ml), and Velcade (Vel; 100 nM) for 24 h. Average values of caspase-8-specific activity are shown from duplicate experiments. (D) Induction of apoptosis was determined by Annexin-V staining (flow cytometry analysis) following treatment with either DZ-50 or doxazosin for 24 h (right panel). Left panel indicates the average percentage of Annexin-V positive cells.

Cont = control.

*p < 0.01.