Table 1.
Pichia pastoris strains used in this study
| Strain | Designation | Genotype (explanation, plasmid used for transformation) | Reference |
|---|---|---|---|
| PPY12 | Wild-type | arg4 his4 | (Sakai et al., 1998) |
| STW1 | GEP-PTS1 | PPY12 his4::PAOX1-GFP-PTS1-HIS4 (pTW51) | (Sakai et al., 1998) |
| SA1017 | YFP-PpAtg8 | PPY12 arg4:: PATG8-YFP-PpATG8 (pSAP115) | Mukaiyama et al. (2003) |
| YAP2401 | Ppatg24Δ[ρ] | PPY12 atg24::ZeocinR (pYA101) | This study |
| YAP2402 | Ppatg24Δ[ρ] | STW1 atg24::ZeocinR (pYA101) | This study |
| YAP2403 | PpATG24/Ppatg24Δ[ρ] | YAP2401 his4::PATG24-PpATG24-HIS4 (pYA101) | This study |
| YAP2404 | PpAtg24-CFP | YAP2401 his4::PATG24-PpATG24-CFP-HIS4 (pYA103) | This study |
| YAP2405 | PpAtg24-CFP/YFP-PTS1 | YAP2403 arg4::PAOX1-YFP-PTS1-ARG4 (pYA005) | This study |
| YAP2406 | PpAtg24-CFP/YFP-2ξFYVE | YAP2403 arg4::PACT1-2 × FYVEHrs-ARG4 (pYA201) | This study |
| YAP2407 | PpAtg24-CFP-PXΔ | YAP2401 his4::PATG24-PpATG24-CFP-PXΔ-HIS4 (pYA104) | This study |
| YAP2410 | YFP-PpAtg8/Ppatg24Δ[ρ] | YAP2401 arg4:: PATG8-YFP-PpATG8-ARG4 (pSAP115) | This study |
| YAP2411 | PpAtg24-CFP/YFP-PpAtg8 | YAP2404 arg4 :: PATG8-YFP-PpATG8-ARG4 (pSAP115) | This study |
| YAP2412 | PpAtg24-CFP/PpVac8-YFP | YAP2404 arg4 :: PVAC8-PpVAC8-YFP-ARG4 (pTN204) | This study |
| YAP2413 | PpAtg24-CFP/YFP-PpAtg17 | YAP2404 arg4 :: PGAP-YFP-PpVAC8-ARG4 (pYA405) | This study |
| YAP2414 | PpAtg24-CFP/Ppvps15 | Ppvps15 his4:: PATG24-PpATG24-CFP-HIS4 (pYA103) | This study |
| YAP2415 | YFP-PpAtg8/CFP-PTS1/Ppatg24Δ[ρ] | YAP2410 his4::PAOX1-CFP-PTS1-HIS4 (pYA006) | This study |
| YAP0004 | YFP-PpAtg8/CFP-PTS1 | SA1017 his4:: PAOX1-CFP-PTS1-HIS4 (pYA006) | This study |