Fig. 2.

GSH, GSSG, Total glutathione, and redox potentials change during development, and recovery from oxidation is stage- and time-dependent. Embryos were exposed to 750 µM tBOOH at specific times of development (24, 48, 72, 96, 120 hpf) and sampled 1, 10, or 60 min following initial exposure. Reduced (GSH) and oxidized (GSSG) glutathione were quantified and total glutathione content and resulting redox potentials were calculated. Asterisks (*) indicate a statistically significant difference between control and tBOOH-treated embryos (p < 0.05). N = 3 pools of 15 embryos for all groups.