Fig. 6.

Nrf2 knockdown impacts glutathione signaling in control embryos but does not confer additional sensitivity to pro-oxidants at 26 hpf. Embryos were injected with Control, nrf2a, or nrf2b morpholinos within 1 hpf. Embryos from each morpholino group were either maintained in media (Control treatment) or tBOOH was added to media (tBOOH treatment) for 2 h prior to sampling. Asterisk (*) indicates a significant difference between untreated control and untreated morpholino-injected embryos (p < 0.05). Octothorpes (#) indicate a statistically significant difference between tBOOH-treated embryos are their matched morpholino untreated controls (p < 0.05). N = 3–5 pools of 10–15 embryos for all groups.