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. 2005 Jan 15;19(2):202–207. doi: 10.1101/gad.328105

Figure 1.

Figure 1.

Depletion of Mad2 results in aneuploidy. (A) Schematic representation of meiosis I in mouse oocytes. See text for further details. (B) Mouse oocytes at the GV-stage (lane 3), at mid-meiosis I (lane 4), and at metaphase II (lane 5) were blotted for Mad2 alongside metaphase II human oocytes (lane 2). Mouse testes lysate (MTL; lane 1) and GAPDH served as positive and loading controls, respectively. (C) Mouse oocytes microinjected with Mad2MO at the GV-stage were blotted for Mad2 after they had completed meiosis I. Note that Mad2 levels in 91 Mad2-depleted oocytes (lane 3) are lower than levels in 51 (lane 4) and 36 (lane 5) wild-type oocytes. MTL (lane 1) and GAPDH served as positive and loading controls, respectively. (D) C-banded spread of a wild-type metaphase II oocyte possessing a normal chromosome compliment of 20 monovalents numbered 1–20. (E) A typical C-banded spread of a hyperploid metaphase II oocyte resulting from Mad2 depletion possessing 20 + one monovalents numbered 1–21. (F) A spread of a Mad2-depleted metaphase II oocyte displaying chromatids. Monovalents are numbered 1–18 and the four chromatids numbered 19a, 19b, 20a, and 20b are indicated by arrows. The total chromosome compliment is equivalent to 20 monovalents (18 monovalents + four chromatids). Chromosome numbering relates to sequential order and not to karyotypic nomenclature. Original magnification, 1000×.