Figure 8.

ATP-2 does not function or localize in the cilia of AWA sensory neurons. (a and b) Confocal micrographs of Podr-10::ATP-2::GFP-(a) and Podr-10::ODR-10::GFP (b)-labeled animals. A 1.0-kb odr-10 promoter was used to drive expression of either ATP-2::GFP or ODR-10::GFP in olfactory AWA sensory neurons of the head. (a) ATP-2::GFP is restricted to mitochondria and absent from the cilium in an AWA neuron. Dashed rectangular boxes show the absence of ATP-2::GFP in the ciliary zone of the AWA neurons. (b) ODR-10::GFP protein is enriched in the cell bodies (arrow) and complex fork-shaped cilia (arrowhead) of AWA neurons. Labeled bars indicate length in micrometers. (c) TS-IR-RNAi of atp-2 or overexpression of ATP-2::GFP does not affect AWA olfactory neuron function. Chemotaxis responses to the odorant diacetyl of the wild-type positive control, the negative control osm-5(p813), and transgenic animals expressing Podr-10::ATP-2::GFP or Podr-10::atp-2-IR RNAi. Chemotaxis assays were performed as described in Sengupta et al. (1996). Diacetyl was diluted 1:1000 in ethanol. Chemotaxis index = (no. animals at the odorant vs. no. animals at the control)/total animals on plate. Approximately 200 animals were used in each assay. Four to six assays were performed for each group. Data are represented as mean ± SEM. Asterick, p < 0.01 compared with wild-type control, Podr-10::ATP-2:GFP, and Podr-10::atp-2-IR RNAi.