FIG. 1.

A, Schematic illustrating the diisopropylfluorophosphate (DFP) exposure paradigm and experimental design. Amongst DFP-exposed rats, 72% survived to 12 h post exposure; of those, 82% survived until euthanized. Imaging and histopathological examinations were performed on 28 DFP-exposed and 7 vehicle control rats at varying days post exposure (DPE) based on their injection group. The number of DFP-injected animals (DFP) and vehicle controls (VEH) imaged per injection group is listed at the end of each row. Note that subsets of animals were imaged at 1–7 days prior to DFP exposure (baseline). B, Scale used to score seizure severity. SLUD = salivation, lacrimation, urination, defecation, which are classic symptoms of cholinergic crisis.