Figure 7.
Localization of UNC-14 depends on kinesin-1. (A) Localization of UNC-14. P unc-25-UNC-14::GFP (kmEx821) was expressed in D-type neurons of wild-type, unc-116(e2281), klc-2(km11), and unc-16(e109) animals. Panels show localization of UNC-14::GFP in the ventral nerve processes of D-type neurons. UNC-14::GFP showed a punctate pattern in wild-type animals (top left). In unc-116(e2281), klc-2(km11), and unc-16(e109) mutant animals, UNC-14::GFP was diffused and irregular along the ventral nerve processes (top right and bottom panels). (B) Localization of KLC-2 and UNC-16 in unc-14 mutants. Punc-25-KLC-2::GFP (kmEx811) and Punc-25-UNC-16::GFP (kmEx823) were expressed in D-type neurons of wild-type and unc-14(ju56) mutant animals. Panels show localization of KLC-2::GFP (left panels) and UNC-16::GFP (right panels) in the ventral nerve processes of D-type neurons. Both KLC-2::GFP and UNC-16::GFP were evenly distributed in wild-type and unc-14(ju56) mutant animals. Scale, 10 μm