Table 2.
UNC-14::GFP expression in kinesin-1 and unc-16 mutants
| Genotype | % Punctate | % Diffuse | n |
|---|---|---|---|
| kmEx820 | 80.7 (71) | 19.3 (17) | 88 |
| kmEx821 | 75.5 (40) | 24.5 (13) | 53 |
| unc-116(e2281); kmEx820 | 23.7 (22) | 76.3 (71) | 93 |
| unc-116(e2281); kmEx821 | 23.6 (13) | 76.4 (42) | 55 |
| klc-2(km11); kmEx820 | 27.8 (25) | 72.2 (65) | 90 |
| klc-2(km11); kmEx821 | 25.5 (13) | 74.5 (38) | 51 |
| unc-16(e109); kmEx820 | 48.8 (41) | 51.2 (43) | 84 |
| unc-16(e109); kmEx821 | 50.0 (26) | 50.0 (26) | 52 |
The UNC-14::GFP localization pattern (from two independent extrachromosomal arrays) was observed by taking confocal images under the same settings. The score “punctate” refers to that UNC-14::GFP was seen as discrete and regularly spaced fluorescent puncta, and “diffuse” refers to that UNC-14::GFP appeared diffuse and were not visually separable as puncta. n, number of animals scored.