Figure 8.

MT organization in growing hyphal tips of wild type and a ΔkipA mutant (see Movies 2-5). (A-C) Wild-type strain SJW02 was grown on glycerol at 25°C. (A) GFP-labeled MTs are displayed in shadow mode in images captured at 3-s intervals. (B) GFP-KipA images captured at 2-s intervals. The disappearance of tip signal in image 5 and its reappearance in image 6 suggests that the maintenance of KipA at the tip requires continuous delivery from the plus ends. (C) Scheme of the traces of GFP-KipA. During an observation time of 4 min, 18 MT plus ends used the same tracks indicated with lines. (D) ΔkipA strain SSK67 was grown on glycerol at 25°C and imaged as in A. (E) Quantification of the effects. Left, data from 157 MTs in 27 germlings of strain SJW02 and from 151 MTs in 29 germlings of strain SSK67. Right, in the same dataset, the movements of MT plus ends away from their meeting point were measured and grouped into short, medium, and long movements.