Table 1.
Analysis of AWC phenotypes
| Strain | 2 AWCOFF (%) | 1 AWCOFF/1 AWCON (%) | 2 AWCON (%) | n |
|---|---|---|---|---|
| Wild type (N2) | 2 | 98 | 0 | 241 |
| Single mutants | ||||
| unc-43(n1186lf) | 1 | 1 | 98 | 180 |
| unc-43(n498gf) | 83 | 17 | 0 | 71 |
| tir-1(ky388ts) | 0 | 42 | 58 | 286 |
| tir-1(tm1111lf) | 0 | 26 | 74 | 80 |
| tir-1(ok1052) | 36 | 50 | 14 | 133 |
| tir-1(gk264) | 0 | 99 | 1 | 175 |
| odr-3::tir-1::DsRed transgenea | 7 | 93 | 0 | 97 |
| odr-3::tir-1(OE) transgeneb | 94 | 6 | 0 | 617 |
| nsy-1(ky542lf) | 0 | 1 | 99 | 156 |
| odr-3::nsy-1(gf) transgeneb | 80 | 20 | 0 | 206 |
| Double mutants | ||||
| tir-1(ky388ts); odr-3::tir-1::DsRed transgene | 0 | 99 | 1 | 107 |
| unc-43(n1186lf); odr-3::tir-1(OE) transgeneb | 90 | 9 | 1 | 248 |
| unc-43(n498gf); tir-1(ky388ts) | 1 | 40 | 59 | 145 |
| unc-43(n498gf); tir-1(tm1111lf) | 1 | 30 | 69 | 115 |
| nsy-1(ky542lf); odr-3::tir-1(OE) transgeneb | 0 | 3 | 97 | 182 |
| tir-1(ky388ts); odr-3::nsy-1(gf) transgene | 85 | 13 | 2 | 257 |
| tir-1(tm1111lf); odr-3::nsy-1(gf) transgeneb | 76 | 24 | 1 | 267 |
Animals were grown at 20°C.
a
The odr-3::tir-1::DsRed transgene was injected at 5 ng/μl.
b
Results were combined from two transgenic lines, which showed similar penetrance of phenotypes. The odr-3::tir-1 transgene was injected at 50 ng/μl.