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. 2005 Feb;16(2):584–596. doi: 10.1091/mbc.E04-09-0768

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Figure 5. — RT-PCR assays of cdc16-I2, erf1-I2, and pyp3-I1 splicing in the ts spU2AF^SM and spU2AF^LG mutants. (A) Top left: gel electrophoretic analysis of products from cdc16-I2 in the spU2AF^SM [Y108A, F111A] mutant. Bottom left: histogram showing quantitation of RT-PCR data with percent precursor mRNA indicated by white bars and percent mature mRNA indicated by black bars. Error bars, SD for three RT-PCR splicing assays. (Right: as in the left panels except that the experiment was performed with the spU2AF^LG C387Y mutant. Bottom: the sequence of the 3′ end of cdc16-I2 with the branchpoint sequence indicated in italics, the pyrimidine tract underlined, and the terminal AG in uppercase. (B) As in A except that the splicing data are for erf1-I2. (C) As in A except that the splicing data are for pyp3-I1.