Table 1.
Meiotic division and meiotic recombination in the xrs2 mutants
Fold increase of recombinant fraction
|
||||
---|---|---|---|---|
Mutants | Spore formation | Spore viability | (24 h/0 h in SPM) | |
(%) | (%) | Arg+ | His+ | |
No tag | 86 | 97 | 600 | 340 |
Empty | 36 | 0 | 1.8 | 1.6 |
ΔC 0 | 88 | 99 | 1600 | 330 |
ΔC 1 | 87 | 100 | 620 | 260 |
ΔC 2 | 87 | 92 | 1300 | 270 |
ΔC 3 | 85 | 86 | 840 | 190 |
ΔC 4 | 47 | 0 | 2.2 | 1.1 |
ΔN 0 | 73 | 76 | 290 | 51 |
ΔN 1 | 18 | 3 | 30 | 22 |
ΔN 2 | 31 | 0 | 24 | 5.8 |
ΔN 3 | 60 | 21 | 140 | 25.1 |
ΔN 4 | 87 | 86 | 930 | 130 |
ΔN 8 | 84 | 88 | 1900 | 290 |
ΔN 11 | 80 | 96 | 470 | 150 |
ΔN 12 | 66 | 18 | 140 | 51 |
ΔN 13 | 82 | 8 | 110 | 53 |
ΔN 14 | 78 | 38 | 110 | 77 |
ΔN 15 | 74 | 54 | 160 | 57 |
ΔN 16 | 87 | 40 | 550 | 110 |
ΔN 17 | 82 | 78 | 100 | 80 |
ΔN 18 | 77 | 96 | 650 | 180 |
ΔN 20 | 69 | 0 | 9.4 | 2.4 |
ΔN 21 | 35 | 0 | 1.3 | 1.1 |
K641E | 46 | 0 | 1.7 | 1.7 |
4AQ | 81 | 100 | 1300 | 240 |
ΔM | 45 | 0 | 2.2 | 1.3 |
E-empty | 46 | 0 | 2.9 | 1.3 |
E 1 | 56 | 3 | 5.0 | 6.9 |
E 4 | 67 | 39 | 76 | 19 |
E 1-K641E | 53 | 0 | 3.6 | 0.8 |
E 4-K641E | 31 | 0 | 4.6 | 2.3 |
Spore formation indicates percentage of cells containing more than one spore in all visible cells after 24 h. Spore viability was measured by dissection of asci by micromanipulation, and the proportion of spores germinating to give visible colonies was assessed. Eighteen asci were dissected for each strain.