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Supplementation with sorbitol supports growth of CL-deficient mutants at 37°C. (A) Isogenic wild-type and pgs1Δ mutant cells from two genetic backgrounds (FGY3 ρ0 and GA74D ρ+) were serially diluted and spotted on YPD or YPDS plates and incubated at the indicated temperature. (B) Wild-type (FGY3) and crd1Δ (FGY2) cells were grown overnight in YPD. Single cells were seeded on YPD or YPDS plates and incubated at the indicated temperature.
