Table 1.
Plasmids and yeast strains used in this study
| Plasmid or strain | Characteristics or genotype | Source or reference |
|---|---|---|
| pYES2/CT | 2 μm, URA3 | Invitrogen |
| pRS415-PGS1 | derivative of pYES2/CT, expresses PGS1 from Gal1 promoter | He and Greenberg (2004) |
| Ycp50 | Centromere, URA3 | Rose et al. (1987) |
| Ycp50-KRE5 | derivative of Ycp50, expresses KRE5 from its own promoter | This study |
| GAD74D3A | MAT α, ade8, ura 3, trp1, his3, leu2 | Dzugasova et al. (1998) |
| GAD74D3C | MAT α, ade8, ura 3, trp1, his3, leu2, pgs1::HIS3 | Dzugasova et al. (1998) |
| FGY3 | MAT α, ura 3-52, lys2-801, ade2-101, trp1Δ1, his3Δ200, leu2Δ1 | Jiang et al. (1997) |
| FGY3 (ρ0) | rho0 mutant derived from FGY3 | This study |
| QZY24B (ρ0) | MAT a, ura 3-52, lys2-801, trp1Δ1, his3Δ200, leu2Δ1, pgs1Δ::TRP1 | This study |
| QZY11A (ρ0) | MAT a, ura 3-52, lys2-801, trp1Δ1, his3Δ200, leu2Δ1, pgs1Δ::TRP1, kre5W1166X | This study |
| FGY2 | MAT α, ura 3-52, lys2-801, ade2-101, trp1Δ1, his3Δ200, leu2Δ1, crd1Δ::URA3 | Jiang et al. (1997) |
| 100 | MAT a, ade1, oxi2 (—) | C. Dieckmann |
| 101 | MAT a, ade1, oxi1 (—) | C. Dieckmann |
| 102 | MAT a, ade1, oxi3 (—) | C. Dieckmann |
| 103 | MAT a, ade1, cob (—) | C. Dieckmann |
| 104 | MAT α, met6, oxi2 (—) | C. Dieckmann |
| 105 | MAT α, met6, oxi1 (—) | C. Dieckmann |
| 106 | MAT α, met6, oxi3 (—) | C. Dieckmann |
| 107 | MAT α, met6, cob (—) | C. Dieckmann |
| T158c/S14a | Diploid prototroph S. cerevisiae | ATCC (46427) |