Figure 6.
Sedimentation of bottom fractions 19-22 in second 10-20-30% iodixanol top-bottom gradient. MDCK cells were grown as confluent monolayers on Transwell filters for 3 d after cell-cell adhesion. After separation in a regular 10-20-30% floating iodixanol gradient, fractions 19-22 were combined with half of fractions 4-9. Membranes were spun from the top of the gradient in a 10-20-30% iodixanol gradient as described Materials and Methods. Signal intensity for each protein band was determined as integrated intensity (counts/mm2) and expressed as percentage of the sum of integrated intensities in fractions 1-25 (see Materials and Methods for details). In the 3-D graph, the y-axis (arbitrary units) is omitted to increase clarity of the graphical display.