Figure 7.

Transmembrane proteins (A and B) and adherens junction proteins (C and D) during formation of the AJC (6 h after cell-cell adhesion). MDCK cells were grown as confluent monolayers on Transwell filters for 6 h after cell-cell adhesion. (A and C) 10-20-30% iodixanol gradient. After separation of fraction samples by SDS-PAGE followed by immunoblotting, signal intensity for each protein band was determined as integrated intensity (counts/mm²) and expressed as percentage of the sum of integrated intensities in fractions 1-25 (see Materials and Methods for details). In the 3-D graph, the y-axis (arbitrary units) is omitted to increase clarity of the graphical display. (B and D) Immunofluorescence. II/III/VI/VII are 3-D reconstructions of confocal z-stacks in a 45°-angled view. I/IV/V/VIII are z-sections of corresponding 3-D reconstructions. Bar, 5 μm.