Figure 3.

Sla2p binds to PtdIns(4,5)P₂ specifically through the ANTH domain. (A) Liposomes made from major-minor lipid mix with 2 mol% PtdIns(4,5)P₂ were tested for binding to GST-Sla2p (0.5 μM), GST only (0.5 μM), and GST-ANTHΔ (0.5 μM) in which the ANTH domain (amino acids 1-125) was deleted. (B) Binding of Sla2p mutants to liposomes made from major-minor lipid mix with 2 mol% PtdIns(4,5)P₂. The protein concentration in each assay is 0.5 μM. K14, K24, K26, and K62 are conserved residues in the ANTH domain (Figure 1A). K42 is not conserved and was mutated as a control.