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(A) Binding domain of rab27a on Munc13-4. Domain organization of Munc13-4 and truncation constructs (A). Binding of 35S-labeled His-Munc13-4 truncations to GTPγS charged GST-rab27a was done as described under Materials and Methods. (B) Analysis of the Munc13-4 truncation mutants indicated that the rab27a binding domain is within the region comprising aa 240–543.
