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Trafficking of Snc1p and Chs3p requires Pik1p and Ypt31p. (A) GFP-Snc1p or (B) Chs3p-GFP distribution at 26°C in live cells, either wild-type (Wt) or carrying the indicated mutation, ypt31Δ, pik1-139ts, and pik1-139ts ypt31Δ. Arrows indicate bud necks and arrowheads indicate bud tips. (B) Chs3p-GFP transport to the cell surface was monitored by blocking endocytosis with LatA (200 μM, Molecular Probes) or treated with vehicle (dimethyl sulfoxide) as described in Valdivia et al. (2002).
