Figure 3.

Trafficking competence of pik1-139^ts ypt31Δ double mutant cells at permissive temperature. (A) Hxt1p-GFP and Tat2p-GFP distribution at 26°C in Wt, ypt31Δ, pik1-139^ts, and pik1-139^ts ypt31Δ. (B) Indicated yeast strains (Wt, ypt31Δ, pik1-139^ts, and pik1-139^ts ypt31Δ) were metabolically labeled with Tran-³⁵S for 10 min, and chased in the presence of excess methionine/cysteine for 30 min at 26°C. CPY and Gas1p were immunoprecipitated from cell lysates, resolved by SDS-PAGE, and visualized by fluorography. p, precursor form; m, mature form.