Figure 8.

Gyp2p is a GAP for Ypt31p in vivo. (A) gyp2 deletion suppresses the temperature-sensitive growth of trs130-HA^ts. (B) SEC4 and YPT6 (high-copy) do not suppress the temperature-sensitive growth defect of trs130-HA^ts. (C) gyp2 deletion suppresses the temperature-sensitive growth of ypt32Δypt31-101^ts double mutant cells. (D) gyp2 deletion specifically rescues trafficking defects of ypt32Δypt31-101^ts double mutant cells. ypt32Δypt31-101^ts, ypt32Δypt31-101^ts gyp1Δ, and ypt32Δypt31-101^tsgyp2Δ were incubated at 37°C for 30 min, metabolically labeled with Tran-³⁵S for 10 min, and chased in the presence of excess methionine/cysteine for the indicated minutes. CPY and Gas1p were analyzed as described in the legend to Figure 3.