Figure 7.

Ln-5 induces expression of osteopontin and osteocalcin in hMSC. (A) RT-PCR was performed on hMSC grown for 16 d on tissue culture plastic in the absence (CTL) or presence of OS media (+OS), or on Ln-5. Where indicated by a “+” symbol, 50 μM PD98059 was added to the culture conditions. Indicated genes were amplified using primers listed in Table 1. GAPDH was amplified as a loading control. (B–E) Densitometric analysis of bands in A for (B) ALP, (C) osteocalcin, (D) osteopontin, and (E) runx2/CBFA-1.