Table 1.

RT-PCR primers

Gene name	Primer sequences	Product size (bp)
ALPL (alkaline phosphatase)	Forward 5′-GGGGGTGGCCGGAAATACAT-3′	543
	Reverse 5′-GGGGGCCAGACCAAAGATAGAGTT-3′
SSP1 (osteopontin)	Forward 5′-AGACCCCAAAAGTAAGGAAGAAGA-3′	564
	Reverse 5′-GACAACCGTGGGAAAACAAATAAG-3′
BGLAP (osteocalcin)	Forward 5′-CGCAGCCACCGAGACACCAT-3′	400
	Reverse 5′-AGGGCAAGGGGAAGAGGAAAGAA-3′
CBFA1 (core binding factor alpha 1)	Forward 5′-ATGGCGGGTAACGATGAAAAT-3′	421
	Reverse 5′-ACGGCGGGGAAGACTGTGC-3′
GAPDH (Glyceraldehyde-3-phosphate dehydrogenase)	Forward 5′-ATGGAAATCCCATCACCATCT-3′	1000
	Reverse 5′-GGTTGAGCACAGGGTACTTTATT-3′
Laminin alpha 1 chain	Forward 5′-ATTGCCTTCCAGCGAAACCGG-3′	780
	Reverse 5′-TTGGACACATTTATCGTCCTG-3′
Laminin beta 1 chain	Forward 5′-TTGGACCAAGATGTCCTGAG-3′	659
	Reverse 5′-CAATATATTCTGCCTCCCCG-3′
Laminin-5 alpha 3 chain	Forward 5′-GTGGTTACCTCACTTACCAAGCCA-3′	269
	Reverse 5′-GGTGAGCCTTTGAGTCTCTGTGAA-3′
Laminin-5 beta 3 chain	Forward 5′-CCAATATCATGCCCTGGTGAGCTA-3′	374
	Reverse 5′-TGCAGAACAGTAGCTGAGTCTGTG-3′
Laminin-5 gamma 2 chain	Forward 5′-TGGTGATTACAGAAGCCAGAAGG-3′	436
	Reverse 5′-GTCAGTTGACCTGAGCATACCCAT-3′