Table 2.
Density of gold particles in flat lattices on CHO-CCR5 cells
| Average ± SD
|
||
|---|---|---|
| Untreated | CCL5 (2 min) | |
| No. of lattices analyzed | 163 | 168 |
| Lattice (% of total membrane) | 1.20 ± 0.28 | 1.08 ± 0.51 |
| Area/lattice (μm2) | 0.05 ± 0.02 | 0.04 ± 0.01 |
| Gold particles/μm2 of lattice | 0.58 ± 0.47 | 8.30 ± 2.89 |
Arbitrarily selected fields of membranes prepared from ripped-open cells were photographed. Equivalent surface areas of membranes from untreated or 2-min CCL5-treated samples were analyzed. The total number of flat lattices found on these membranes was similar. The area of membrane covered by lattice is expressed as % of total membrane area analyzed. The average size of a lattice and the average density of gold particles per square micrometer of lattice were determined as described in Materials and Methods.