Figure 2.

Effects of benomyl on hyphal tip growth. (A) Time-lapse images of a GFP-α-tubulin–expressing strain from Fig 2.mov. Images are projections of Z-stacks obtained with a wide-field microscope and the time (in seconds) after the start of observation is indicated in the upper right corner of each panel. Benomyl was added 900 s after the start of observations. The hypha at the left was in interphase and the hypha at the right was in anaphase when benomyl was added. A mitotic spindle is visible in the hypha at the right at the 840-s time point. The growth of both hyphae dramatically slowed after the addition of benomyl. Bulging of tips after benomyl addition is indicated by arrows, and bulging of a medial region of the hypha at the left is indicated by an arrowhead. (B) Profile of the growth of the left hypha in A. The time of benomyl addition is designated with the filled arrow and the completion of cytoplasmic microtubule disassembly is designated by the open arrow. The rates of tip growth before and after the addition of benomyl were determined by regression analysis (gray lines) and are indicated.