Figure 3.

Growth recovery after benomyl washout. A rapidly growing hypha was treated with benomyl for 15 min. The benomyl was then washed out and tip growth was monitored. (A) The hyphal tip just before benomyl addition. (B) The same hyphal tip after 15 min in benomyl and immediately before benomyl washout. The microtubules have disassembled. (C) Time course images (Fig 3.mov) of the same hypha after benomyl washout. Imaging was restarted as soon as possible after benomyl washout (time 0). Microtubules are clearly visible at 540 s, and there is a visible bulge in the hyphal tip (arrowhead). The bulge is larger than before the benomyl washout. Normal tip growth has resumed by the 1440-s time point, and the hyphal bulge is still visible (arrowhead). (D) The growth of the hyphal tip was plotted against time after the completion of benomyl washout. Tip growth accelerated after the benomyl washout and reached a consistent rate of 0.458 μm (gray line, determined by regression analysis).