Figure 4.

The temperature-sensitive mtg2-1 mutant displays aberrant mitochondrial translation. (A) The growth of mtg2-1 cells on YPG is impaired at 37°C. Shown are serial dilutions of cells expressing MTG2 or mtg2-1 on YPD and YPG plates at 23°C and 37°C, as indicated. (B) Newly synthesized mitochondrial protein products were labeled in vivo in cells expressing MTG2 or mtg2-1, with [³⁵S]methionine, at either 23°C or after shift to 37°C for either 2 or 17 h. Mitochondria were isolated, and equivalent amounts of protein were separated by SDS-PAGE, the gel dried, and the radioactive proteins visualized on x-ray film. The position of Cox1p, Cox2p, Cytbp, and Cox3p is indicated.