Figure 6.

The stability of early assembling large ribosomal proteins is not affected in mtg2–1. Mitochondrial protein extracts obtained from cells expressing MTG2 or mtg2–1 allele grown at either 23°C or 37°C (for 2 or 17 h), as indicated, were separated by SDS-PAGE and subjected to immunoblot analysis. The relative levels of Mtg2p, the early assembling proteins Mrp49p and Mrp13p, and the late assembling protein Mrp7p were detected using protein specific antibodies. Loading controls include Cox2p and Tim23p, two proteins that should remain unaffected in this assay.