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The levels of intracellular ROS were detected by flow cytometry. (A) ROS in HUVECs that were treated with different concentrations of glucose (5.5 mM, 33 mM) was analyzed after 24 h by flow cytometry. (B) Data of ROS expression were expressed as mean ±SD of 4 independent groups. The superscript letter on columns represented that * P<0.05 compared with control group.
