Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Effect of GTPγS on the Mn2+-dependent sGC activity. gca- cells were lysed in the presence or absence of 100 μM GTPγS. Lysis was performed at either 1 μM free Ca2+ or 1.5 mM EGTA. Lysates were fractionated by centrifugation, and activities were measured in pellets (resuspended in LB, filled bars) and supernatants (open bars on top of filled bars). The activity in the pellet with GTPγS is not significantly different in a Student's t test when compared with the pellet without GTPγS.
