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. 2005 Feb;16(2):997–1010. doi: 10.1091/mbc.E04-10-0895

Figure 3.

Figure 3.

Thawed frozen section EM-immunocytochemistry by using antibodies Ab-1 and Ab-5 on human breast cells, HeLa cells, and normal primary human cells obtained by nasal brushing. BRCA1 gold clusters (arrowheads) in nuclei of HBL100 (1), HeLa (2), HMEC (3), and normal primary human cells (4) by using anti-BRCA1 antibodies Ab-1 (1–3) and Ab-5 (4). In normal primary human cells the clusters are only visible in the lighter euchromatin regions or at the boundary of eu- and heterochromatin (4). Bars, 500 nm (1–4). C, cytoplasm; Eu, euchromatin; He, heterochromatin; Ne, nuclear envelope; N, nucleus; and n, nucleolus.