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. 2005 Feb;16(2):997–1010. doi: 10.1091/mbc.E04-10-0895

Figure 7.

Figure 7.

Western blotting of both untreated and treated NF, CF, MitF, and WH HeLa samples with p-BRCA1 Ser 988, p-BRCA1 Ser 1497, Ab-4, and Ab-C. (A) p-BRCA1 Ser 988 and p-BRCA1 Ser 1497 on MitF HeLa fractions (1) and WH (2) give a 220-kDa BRCA1 band in both lanes. (B) Western blotting with Ab-C and Ab-4 on NF and CF before (1) and after (2) dephosphorylation with λ protein phosphatase. The 220-kDa band disappeared after λ protein phosphatase treatment, showing that it is this species that is phosphorylated. (C) Western blotting with Ab-C on mitochondrial fractions treated for enzyme protection assay. Mitochondrial HeLa untreated control fraction (1), treated with TX100 (2), treated with λ protein phosphatase (3), and treated with λ protein phosphatase and TX100 (4) are presented (top). The 220-kDa BRCA1 band only disappears after combined λ protein phosphatase and TX100 treatment. Mitochondrial HeLa untreated control fraction (1), treated with proteinase K (2), and treated with proteinase K and TX100 (3) are presented (bottom). The 200-kDa band disappears after proteinase K treatment alone, whereas the 220-kDa band only becomes vulnerable after combined proteinase K and TX100 treatment.