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. 2005 Feb;16(2):997–1010. doi: 10.1091/mbc.E04-10-0895

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Copyright © 2005, The American Society for Cell Biology

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Figure 8. — Biochemical analysis of rat liver mitoplasts. (A) Antibody-5 anti-BRCA1 antibody detects the protein in WH, MitF, and a MP-enriched fraction (arrowhead). After dephosphorylation, the top BRCA1 band is strongly reduced, whereas a lower band occurs (arrow). P-BRCA1 Ser 1497 anti-BRCA1 antibody detects the same protein in WH, MitF, and the MP-enriched fraction. After dephosphorylation, all p-BRCA1 Ser 1497 signal is absent. (B) Western blot of Percoll-purified IM and MP stained with Ab-5 showed an increase in signal for two hyperphosphorylated isoforms (^* and ^**) after MP preparation from IM. Arrowhead, 160 kDa. Table of enrichment shows the MP/IM ratio for CCO and MLDH enzymes and the two asterisked BRCA1 protein isoforms. The graph displays the intensity of the BRCA1 protein forms versus the relative migration. Two hyperphosphorylated BRCA1 proteins (^* and ^**) are enriched in the MP fraction versus IM fraction.