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. 2017 May 31;13:1744806917709372. doi: 10.1177/1744806917709372

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© The Author(s) 2017

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 7. — Ryk activation after SNL surgery induced increased secretion of CCL2 through CaMKII/ PKCγ pathway. (a) Immunohistochemistry shows the expression of Ryk (red) and Iba-1(green) seven days after SNL surgery with and without the administration of anti-Ryk. (Scale bar, 100 µm). (b) Western blot analysis of CCL2, phospho-CaMKII, and phospho-PKCγ for proteins harvested from spinal cord seven days after surgery. Data are shown as mean ± SEM. *p < 0.05, ***p < 0.001 versus control in the corresponding group. (c) Immunohistochemical staining showed that in spinal cord with SNL surgery, Ryk (red) highly expressed area could attract microglia invasion (Iba-1, green), which is lamina II area of the dorsal horn. For microglia (Iba-1 positive) in spinal cord, blocking Ryk receptors with anti-Ryk results in the reduction of microglia invasion into lamina II area of the dorsal horn on post-operative day 7.