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Hepatic tissue injury. Nuclei of lethaly injured hepatocytes were labelled in vivo with propidium iodide (PI). PI-labelled nuclei were quantified using fluorescence intravital videomicroscopy with a 510 to 560 nm excitation and an emission barrier filter greater than 590 nm. PI-labelled hepatocytes were expressed as number of cells/10-1mm3. * p < 0.001 vs. sham; # p < 0.001 vs. 3.0 h I/R; ## p < 0.01 vs. 6.0 h I/R; Data expressed as Mean + 2SD; n = 7 for each group.
