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. 2017 Jun 5;12(6):e0178931. doi: 10.1371/journal.pone.0178931

Fig 8. Quantification of the number of foci present after treatment with compounds 1–3 and 2–5.

Fig 8

(A) Quantification of foci per cell from the analysis of a minimum of 1000 DM1 fibroblast nuclei in a range of four log dilutions starting at 40 μM for 2–5 and 100 μM for 1–3. The results were normalized to DM1 cells treated with 1% DMSO (marked as a red line). (B) Representative fluorescence in situ hybridization (FISH) images showing foci in DM1 fibroblasts after 48 h treatment with a range of four log dilutions starting at 40 μM for 2–5 and 100 μM for 1–3. 1% DMSO and 4 μM ChA3 were used as a negative and positive control, respectively. Nuclei were stained with Hoechst 33342 (blue) and rCUGexp RNA foci were detected with a Cy3-labelled probe (red). Images included 2x enlargement of selected nucleus.