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. Author manuscript; available in PMC: 2018 Feb 1.
Published in final edited form as: Nat Rev Drug Discov. 2016 Dec 23;16(2):89–100. doi: 10.1038/nrd.2016.238

Figure 2. CRISPR-Cas in the generation of cellular models and large-scale screens.

Figure 2

CRISPR-Cas gene editing can be used to generate isogenic cell lines for drug target validation, mechanistic analysis and patient stratification studies. Isogenic cell lines can also be used to generate organoids, which are particularly useful for modelling differentiation and self-organization processes. Large-scale sgRNA libraries can be used for high-throughput pooled or high-content arrayed screens, either on unmodified or CRISPR-Cas-edited cell lines.