Fig 2. E. coli production of 1-propanol is AdhE-dependent and uses threonine as a precursor.
(A) Comparison of HS-SPME/GC-MS analysis of 1-propanol emitted by E. coli WT and ΔadhE mutant biofilm grown for 24 h in LB medium under standard biofilm conditions (see also S2 Fig). (B) HS-SPME/GC-MS analysis of 1-propanol emitted from E. coli WT biofilms formed after 48 h in M9 glycerol minimal medium with or without 0.4% threonine. (C) HS-SPME/GC-MS analysis of 1-propanol emitted from E. coli WT and ΔadhE mutant biofilms formed in LB medium with or without 0.4% threonine.