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. 2017 Jun 5;12(6):e0178925. doi: 10.1371/journal.pone.0178925

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© 2017 Brun et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 2 — Left: Graph shows the percentage of HCT116 cells with HA-p21 present in the cytoplasm determined by immunofluorescence analysis using anti-HA (Roche). Representative images are shown in S3A Fig. 48 hours after transfection with non-targeting (siNT), UBC9 (siUBC9), or SENP2 (siSENP2) siRNAs, cells were transfected with HA-p21 plasmid and 24 hours after treated (Adr) or not (Ctrl) with Adr for 12 hours. Data are the average of 3 different experiments and in each one at least 200 HA-p21 transfected cells were counted per condition. Rigth: Western Blotting to detect UBC9 or SENP2 levels in non-depleted or depleted-HCT116 cells; (-): correspond to cells after 48h of transfection with siRNA and priori to HA-p21 transfection.