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. 2017 Jun 5;12(6):e0179078. doi: 10.1371/journal.pone.0179078

Fig 2. Immortalized MEPM cells maintain migration ability.

Fig 2

(A-B’) Primary (A,A’) and immortalized (B,B’) MEPM cells migrate comparable distances following scratching on a fibronectin substrate. Dashed lines indicate the scratch boundary at 0 hours. Black arrowheads denote farthest migration on either side of the scratch. Solid rectangles indicate the frame of the following panel. (C-D”) Expression of phalloidin (red; C,D) and paxillin (green; C’,D’) as assessed by immunofluorescence analyses in passage 1 primary (C-C”) and passage 14 immortalized (D-D”) MEPM cells. Both primary and immortalized MEPM cells at the leading edge display filamentous actin arcs and focal adhesion formation. Nuclei were stained with DAPI (blue; C”,D”). (E-F) Crystal violet staining of primary (E) and immortalized (F) MEPM cells following migration through a porous membrane. Scale bars, 100 μm. (G) Bar graph depicting integrated densities revealing comparable migration of passage 2 primary and passage 23 immortalized MEPM cells in Transwell assays. Data are presented as mean ± SEM. n.s., not significant.