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. 2017 Jun 5;12(6):e0179078. doi: 10.1371/journal.pone.0179078

Fig 3. Expression of palatal shelf transcripts is similar between primary and immortalized MEPM cells.

Fig 3

Bar graph depicting qRT-PCR values revealing reduced expression of Msx1, Meox2 and Alx3 in passage 13 immortalized MEPM cells as compared to their passage 1 primary counterparts and similar expression of Osr2, Pdgfra, Shox2, Efnb1, Mn1, Tbx22 and Shh across the two cell types. qRT-PCR values are normalized to B2m. qRT-PCR data are presented as mean ± SEM. n.s., not significant. *, p<0.05. E13.5 primary MEPM RNA-sequencing FPKM (fragments per kilobase of transcript per million mapped reads) values [12] are normalized to B2m and E13.5 palatal shelf RNA-sequencing RPKM (reads per kilobase of transcript per million mapped reads) values (www.facebase.org, accession FB00000278) [33] are represented as an average of normalization to Actb, Hsp90ab1 and Ppia.