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. 2012 Dec;47(6):729–737. doi: 10.1165/rcmb.2012-0057OC

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Copyright © 2012 by the American Thoracic Society

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Figure 1. — Effect of 24-hour prostaglandin (PG)E2 (0.1 μM) pretreatment on acute PGE2 inhibitory signaling in human fetal lung (HFL) fibroblasts. (a) PGE2-mediated (0.1 μM) chemotaxis after 24-hour PGE2 pretreatment. (b) PGE2-stimulated cAMP accumulation in HFL fibroblasts pretreated with PGE2 (0.1 μM) for 24 hours. (c) PGE2-mediated chemotaxis after 24-hour E prostanoid receptor (EP) agonist (Ag) (1 μM) pretreatment. (d) PGE2-mediated chemotaxis after 24-hour PGE2 pretreatment in the presence of individual EP receptor antagonists (Ant). (e) PGE2-mediated cAMP accumulation after 24-hour PGE2 pretreatment in the presence of individual EP receptor antagonists. *P < 0.05; **P < 0.01. Bars are means ± SEM; n = 3 separate experiments in all panels.