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Evaluation of the mechanism mediating attenuation of acute PGE2 signaling after 24-hour PGE2 pretreatment. (a) Concentration response of forskolin (FSK)-mediated inhibition of HFL chemotaxis after 24-hour PGE2 pretreatment. (b) Concentration response of FSK-stimulated cAMP accumulation after 24-hour PGE2 pretreatment. *P < 0.05; **P < 0.01. Bars are means ± SEM; n = 3 separate experiments in all panels.
