Figure 8.

N-Acetylcysteine and EUK-134 attenuate Baltimore PM-induced NF-κB activation. HBEpCs grown in 35-mm dishes to approximately 90% confluence were pretreated with varying concentrations of N-acetylcysteine (NAC) (0.05, 0.5, and 5 mM) or EUK-134 (5 μM) for 1 hour. In A and B, cells were challenged with vehicle or vehicle plus Baltimore PM (100 μg/ml) for 15 minutes, and cell lysates (20 μg proteins) were subjected to SDS-PAGE and Western blotted with anti–phospho-IkB and actin antibodies. Shown are representative blots and quantitative analyses from three independent experiments (mean ± SD). *P < 0.05 versus vehicle; **P < 0.05 versus PM challenge. In C, HBEpCs grown on glass coverslips (∼ 95% confluence) were pretreated with NAC (5 mM) for 60 minutes, cells were challenged with vehicle or vehicle plus Baltimore PM (100 μg/ml) for 15 minutes, and cells were washed, fixed, permeabilized, probed with anti-p65 antibody, and examined by immunofluorescence microscopy using a ×60 oil objective. Shown is a representative image from several independent experiments.