TABLE 1.
DONOR DEMOGRAPHICS OF PHAEC CULTURES USED FOR IMMUNOBLOTTING OF p63 ISOFORMS
| Donor | Sex | Age (yr)* | Asthma Status† | Sample Type | Cause of Death |
|---|---|---|---|---|---|
| N1 | F | 26 | None; PC20 > 8.0 | Bronchial brushing | |
| N2 | M | 20 | None | Donor lung | Head trauma |
| N3 | F | 23 | None; PC20 > 8.0 | Bronchial brushing | |
| N4 | F | 47 | None | Donor lung | Head trauma |
| N5 | M | 29 | None; PC20 > 8.0 | Bronchial brushing | |
| A1 | F | 23 | PC20 = 0.757 | Bronchial brushing | |
| A2 | F | 23 | PC20 = 0.25 | Bronchial brushing | |
| A3 | M | 11 | Diagnosed; age 2 years | Donor Lung | Anoxia; asthma |
| A4 | F | 29 | PC20 = 0.42 | Bronchial brushing | |
| A5 | M | 25 | Diagnosed; age 11 years | Donor lung | Anoxia; asthma |
Definition of abbreviations: A, asthmatic; F, female; M, male; N, nonasthmatic; PC20, provocative concentration of methacholine causing a 20% fall in FEV1; PHAEC, primary human airway epithelial cell.
*
Unpaired, two-tailed t test confirmed no significant difference in mean age between disease groups (P = 0.267).
†
For cells obtained by bronchial brushing, methacholine challenge data either confirmed (PC20 < 1.0 mg/ml) or excluded (PC20 > 8.0 mg/ml) a diagnosis of asthma.