TABLE 3.

EPITHELIAL GENE EXPRESSION ARRAY OVERVIEW: FOLD CHANGE IN GENE EXPRESSION AFTER 48-HOUR TREATMENT WITH SIRNA TO ΔN OR α/β p63 ISOFORMS

Gene	ΔN siRNA		α/β siRNA
	Fold Change ± SEM*	P Value†	Fold Change ± SEM	P Value
CTNNB1	0.71 ± 0.04	< 0.001	0.64 ± 0.06	< 0.001
LAMC2	0.46 ± 0.07	< 0.001	0.68 ± 0.05	< 0.01
TJP1	0.62 ± 0.02	< 0.001	0.62 ± 0.07	< 0.001
CD44	0.68 ± 0.05	< 0.01	0.64 ± 0.08	< 0.001
EGFR	0.40 ± 0.15	< 0.01	0.50 ± 0.06	< 0.05
EP300	0.66 ± 0.06	< 0.01	0.54 ± 0.07	< 0.001
F11R/JAM1	0.57 ± 0.07	< 0.01	0.56 ± 0.07	< 0.001
GSK3B	0.74 ± 0.05	< 0.01	0.81 ± 0.06	< 0.01
PLEC1	0.50 ± 0.08	< 0.05	0.50 ± 0.10	< 0.05
CCND1	0.84 ± 0.06	< 0.05	0.77 ± 0.11	< 0.01
JAG1	0.48 ± 0.10	NS	0.39 ± 0.09	< 0.05
CDKN1A	0.86 ± 0.07	NS	0.91 ± 0.08	NS
CEBP	1.14 ± 0.18	NS	0.92 ± 0.09	NS
KRT18	0.68 ± 0.07	NS	0.69 ± 0.10	NS
KRT5	0.57 ± 0.07	NS	0.52 ± 0.13	NS
CLDN1‡	0.95 ± 0.05	< 0.001	0.66 ± 0.08	P < 0.001
NOTCH2‡	0.83 ± 0.04	< 0.001	0.75 ± 0.06	P < 0.001
BMP7	Low expression	Low expression
EGF	Low expression	Low expression
FGFR2	Low expression	Low expression
WNT1	Low expression	Low expression

Definition of abbreviations: ΔN, N-terminally truncated; NS, no significance; siRNA, small interfering RNA.

^*Values expressed represent fold change ± SEM, relative to untreated control samples (n = 5 donors).

^†One-way ANOVA, followed by the Dunnett post hoc test, was used to assess statistical significance versus scrambled siRNA construct.

^‡Indicates genes that were significantly altered by scrambled siRNA treatment versus untreated control samples.