Figure 6. MR1T cell clones exhibit divergent transcriptional responses to antigen stimulation.
Betweeness centrality analysis illustrating the key transcription factors that were differentially expressed in resting (No Ag) and antigen-activated (Ag Act) MR1T cell clones (A) DGB129 and (B) DGB70. Color code represents betweeness centrality score. The size of the nodes (or hubs) indicates the relative importance of individual transcription factors within the whole gene network. (C) Heat-map comparing key transcription factors that were differentially expressed in resting (Rest.) and antigen-activated (Act.) DGB129 and DGB70 MR1T cell clones (analysis performed by PageRank algorithm).
Figure 6—figure supplement 1. FACS analysis of resting and activated DGB129 and DGB70 MR1T cells used for transcriptome studies.
Dot-plots display the expression of CD25 and CD137 activation markers on T cells cultured alone (Resting) or stimulated with A375-MR1 cells (Ag stimulated). Purity of CD25+CD137+ cells sorted from the Ag stimulated groups (Sorted Activated) is shown. Plots are gated on CD3+ DAPI- cells. Resting and Sorted Activated DGB129 and DGB70 MR1T cells were utilized for next-generation sequencing experiments.