FIGURE 5.

During later stages of lymph node development, nestin predominantly labels endothelial cells. (A) Graphs showing the absolute number of GFP⁻ and GFP⁺ cells of nes-creER c ROSA26-GFP mice that received tamoxifen at day 17 after birth and were analyzed 4 wk later. (B) Graphs showing the distribution of GFP⁺ cells among the various lymph node stromal populations. (C–G) Immunofluorescence analysis of nes-creER × ROSA26-GFP mice that received tamoxifen at p17 and were analyzed 4 wk later. (C–F) Lymph node sections stained for gp38 (red) and CD31 (blue) showing GFP⁺ HEVs (C), GFP⁺ capillaries (D), GFP⁺ gp38⁺CD31⁻ stromal cells (indicated with asterisks, E–G), and GFP⁺gp38⁺ CD31⁺ lymphatic endothelial cells (indicated with arrows, G). Images are representative of lymph nodes of five individual animals (A and B) or three individual mice (C–G). Scale bars, 25 μm.