Table I.
The primers used for the RT-PCR in our study.
| Gene | Primer’s sequence | Product size (bp) | Annealing temprature (°C) |
|---|---|---|---|
| Ghrelina | 5′-AGCCTCCTGCTCCTCGGCAT-3′ | 339 | 62 |
| 5′-TGTGGGCGATCACTTGTCGGCT-3′ | |||
| GHS-R1ab | 5′-TCGTGGGTGCCTCGCT-3′ | 65 | 59 |
| 5′-CACCACTACAGCCAGCATTTTC-3′ | |||
| GHS-R1ba | 5′-TGGAGCACGAGAACGGCA-3′ | 304 | 62 |
| 5′-AGGCACAGGGAGAGGATAGGA-3′ | |||
| PYY | 5′-GGACAGGCTTCTTTCCAAAACG-3′ | 158 | 62 |
| 5′-TTCTGGGGTCGGGAGTGCGTATGC-3′ | |||
| NPY2R | 5′-GCTGGCTAATCATCGGACAGAC-3′ | 140 | 60 |
| 5′-GCACCTATTGGACCCATTTTCAG-3′ | |||
| SNRPN | 5′-CGCTTACACCTGAGACGAACTACAG-3′ | 818 | 58 |
| 5′-TGGAGCCTGGTTTTTGCTTG-3′ | |||
| GAPDH | 5′-TGACAACTTTGGTATCGTGGAAGG-3′ | 130 | 58 |
| 5′-AGGGATGATGTTCTGGAGAGCC-3′ |
a
The primers for ghrelin and GHS-R1b were synthesized according to the sequence reported by Carraro et al. (3).
b
The primers for GHS-R1awere synthesized according to the sequence reported by Korbonits et al (12). The primers for the other genes were designed using sequence data available from Genbank.